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RNA-Seq Data Analysis

Learn the complete RNA-Seq analysis pipeline from raw reads to differential gene expression. Covers quality control, alignment, quantification, and downstream functional analysis.

RNA-Seq Data Analysis

Syllabus

RNA-Seq foundations2 lessons
  1. What RNA-Seq Measures10 min
  2. Experimental Design and Replicates12 min
Quality control and read prep2 lessons
  1. Quality Control with FastQC11 min
  2. Adapter and Quality Trimming11 min
Alignment and quantification4 lessons
  1. Alignment vs Pseudo-alignment11 min
  2. Aligning Reads to a Reference11 min
  3. Quantifying Gene Counts11 min
  4. Building the Count Matrix10 min
Differential expression and interpretation5 lessons
  1. Why Raw Counts Mislead: Normalization11 min
  2. Differential Expression with DESeq212 min
  3. Visualizing Results: Volcano and MA Plots12 min
  4. Functional and GO Enrichment12 min
  5. Wrap-up and Where to Go Next10 min